Reliable Activity Even After 1 Month At Room Temperature
Key Features
- 99% active after 1 month at room temperature
- Ships at ambient temperature – no dry ice
- Ideal for automated high throughput applications
- Survive freezer breakdowns and power outages
- Freeze/thaw up to 50 times
Description
5x TUFF TAQ™ SUPERGREEN QPCR Master Mix is engineered with a proprietary peptide tag that confers extreme stability at room temperature. The 5 x mix is optimized for real-time QPCR assays & contains all components required for QPCR except for template and primers.TUFF TAQ™ DNA Polymerase is activated by a 15 min incubation step at 95°C.
Detail
SUPERGREEN Dye is a DNA binding dye with similar spectra to SYBR Green I and is compatible with all common real time cyclers – simply select the standard settings for SYBR Green or FAM
Applications
- Detection & quantification of DNA & cDNA targets
- Gene expression profiling
- Microbial dectection
- Viral load determination
5x Master Mix Composition
- TUFF TAQ™ DNA Polymerase
- 5x QPCR buffer
- dNTPs including dTTP to improve sensitivity & efficiency compared to dUTP 5X SUPERGREEN Dye
- 12.5 mM MgCL2 (1 x working concentration = 2.5 mMMgCL2)
Ordering Information
|
Cat # |
Pack Size |
Conc. (MgCl2) |
|
30-04-0250 |
1 ml (250 reactions) |
15 mM |
|
30-04-5000 |
20 ml (5000 reactions) |
15 mM |
| 30-04-CUST | CUSTOM ORDERS |
Storage & StabilityContact us for bulk discounting, institutional pricing & custom packaging.
- Shipping and temporary storage for up to 1 month at room temperature has no significant detrimental performance effects
- Routine storage: -20°C
Usage restrictions
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Rebel BioScience products may not be re-sold, transferred, modified or used to manufacture commercial products without written approval from Rebel BioScience, Inc.